Anti-PHLDA1 antibody
| 英文名称 | PHLDA1 |
| 中文名称 | T淋巴细胞凋亡相关蛋白TDAG51抗体 |
| 别 名 | Apoptosis associated nuclear protein; Apoptosis-associated nuclear protein; DT1P1B11; MGC131738; PHLA1_HUMAN; PHLDA1; PHRIP; Pleckstrin homology like domain family A member 1; Pleckstrin homology-like domain family A member 1; PQ rich protein; PQ-rich protein; PQR protein; Proline- and glutamine-rich protein; Proline- and histidine-rich protein; T cell death associated gene; T CELL DEATH ASSOCIATED GENE 51; T-cell death-associated gene 51 protein; Tdag; TDAG51. |
DATASHEET
Host:Rabbit
Target Protein:PHLDA1
IR:Immunogen Range:251-360/401
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:22822
Swiss Prot:Q8WV24
Source:KLH conjugated synthetic peptide derived from human PHLDA1:251-360/401
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:PHLDA1 is involved in regulation of apoptosis induced by signaling of T cell receptor in murine lympocytes. As any potent regulator with pleiotropic effects related to its overexpression, PHLDA attracts attention in both cell biology research and human tissue pathology.
Size:100ul
Concentration:1mg/ml
Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Cow
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PHLDA1) Polyclonal Antibody, Unconjugated (bs-1364R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: mouse pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PHLDA1/TDAG51 Polyclonal Antibody, Unconjugated(bs-1364R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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