Anti-Serum albumin(Serum Loading Control) antibody (bs-0945R)

Anti-Serum albumin(Serum Loading Control) antibody

DATASHEET

Host:Rabbit

Target Protein:Serum albumin(Serum Loading Control)

IR:Immunogen Range:

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:213

Swiss Prot:P02768

Source:human Serum albumin puritied: 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin. [provided by RefSeq, Jul 2008].

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Goat
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Lane 1: Serum (Human) Lysate at 20 ug
Lane 2: Serum (Mouse) Lysate at 20 ug
Lane 3: Serum (Rat) Lysate at 20 ug
Lane 4: Serum (Goat) Lysate at 20 ug
Lane 5: Liver (Rat) Lysate at 40 ug
Primary: Anti-Serum albumin (bs-0945R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 67 kD
Observed band size: 67 kD

Sample:HepG2 Cell (Human) Lysate at 40 ug
Primary: Anti-Serum albumin (bs-0945R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 69 kD
Observed band size: 69 kD