Anti-APCDD1 antibody (bs-1565R)

Anti-APCDD1 antibody

DATASHEET

Host:Rabbit

Target Protein:APCDD1

IR:Immunogen Range:30-130/514

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:147495

Swiss Prot:Q8J025

Source:KLH conjugated synthetic peptide derived from human APCDD1:30-130/514 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:APCDD1 is a novel protein that has been shown to be a target of Wnt/beta catenin signaling pathway in cancer cell lines. APCDD1 is overexpressed in colorectal carcinogenesis and is deregulated in CTNNB1 mutated Wilms tumors. [FUNCTION] Probably plays a role in colorectal tumorigenesis. May be a developmental target gene of the Wnt/Beta-catenin pathway.
[SUBCELLULAR LOCATION] Membrane; Single-pass type I membrane protein (Potential).
[TISSUE SPECIFICITY] Abundantly expressed in heart, pancreas, prostate and ovary. Moderately expressed in lung, liver, kidney, spleen, thymus, colon and peripheral lymphocytes.
[INDUCTION] Transcriptionally regulated by the CTNNB1/TF7L2complex.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample: DU145 Cell (Human) Lysate at 40 ug
Primary: Anti-APCDD1 (bs-1565R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD

Images provided the Independent Validation Program (badge number 029637)Formalin-fixed and paraffin embedded human stomach labeled with Rabbit Anti-APCDD1 Polyclonal Antibody (bs-1565R) at 1:250 room temperature overnight followed by conjugation to secondary antibody.