Anti-Cathepsin K antibody
| 英文名称 | Cathepsin K |
| 中文名称 | 组织蛋白酶K抗体 |
| 别 名 | Cathepsin K; Procathepsin K; Cathepsin K precursor; Cathepsin O; Cathepsin O1; Cathepsin O2; Cathepsin X; CathepsinK; CTS02; CTSK; CTSK protein; CTSO; CTSO1; CTSO2; MGC23107; PKND; PYCD; Pycnodysostosis; cath-K; CK. |
DATASHEET
Host:Rabbit
Target Protein:Cathepsin K
IR:Immunogen Range:112-210/329
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:1513
Swiss Prot:P43235
Source:KLH conjugated synthetic peptide derived from human Cathepsin K:112-210/329
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:The protein encoded by this gene is a lysosomal cysteine proteinase involved in bone remodeling and resorption. This protein, which is a member of the peptidase C1 protein family, is predominantly expressed in osteoclasts. However, the encoded protein is also expressed in a significant fraction of human breast cancers, where it could contribute to tumor invasiveness. Mutations in this gene are the cause of pycnodysostosis, an autosomal recessive disease characterized by osteosclerosis and short stature. This gene may be subject to RNA editing. [provided by RefSeq, Jul 2008].
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Guinea Pig
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample: Bone (Rat) Lysate at 40 ug
Primary: Anti-Cathepsin K (bs-1611R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 34 kD
Tissue/cell: mouse heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Cathepsin K Polyclonal Antibody, Unconjugated(bs-1611R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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