Anti-EAAT2 antibody (bs-1751R)

Anti-EAAT2 antibody 

DATASHEET

Host:Rabbit

Target Protein:EAAT2

IR:Immunogen Range:101-200/574

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:6506

Swiss Prot:P43004

Source:KLH conjugated synthetic peptide derived from human EAAT2:101-200/574 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene encodes a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Mutations in and decreased expression of this protein are associated with amyotrophic lateral sclerosis. Alternatively spliced transcript variants of this gene have been identified. [provided by RefSeq, Sep 2010]

Size:100ul

Concentration:1mg/ml

Applications:Flow-Cyt(1μg /test)

Cross Reactive Species:Human
Mouse
Rat
Cow
Horse
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Black line : Positive blank control (HepG2); Negative blank control ( K562）
Green line : Primary Antibody (Rabbit Anti-EAAT2 antibody (bs-1751R) )
Orange line：Isotype Control Antibody (Rabbit IgG) .
Blue line : Secondary Antibody (Goat anti-rabbit IgG-FITC)
HepG2（Positive）and K562（Negative control）cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with EAAT2 Antibody(bs-1751R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Blank control (Black line): U87MG (Black).
Primary Antibody (green line): Rabbit Anti-EAAT2 antibody (bs-1751R-FITC)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-FITC .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.

Cell: H-4-II-E
Concentration:1:100
Host/Isotype:Rabbit/IgG
Flow cytometric analysis of Rabbit IgG isotype control (Cat#: bs-1751R) on H-4-II-E(green) compared with control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG(H+L) secondary antibody .