Anti-GPR14 antibody (bs-1806R)

Anti-GPR14 antibody

DATASHEET

Host:Rabbit

Target Protein:GPR14

IR:Immunogen Range:101-200/389

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:2837

Swiss Prot:Q9UKP6

Source:KLH conjugated synthetic peptide derived from human UTS2R:101-200/389 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:GPCR GPR14 has been reported to be expressed at high levels in the heart and pancreas and also in artery and smooth muscle. Low transcript levels have been identified in brain, particularly in thalamus, superior occipital gyrus, and substantia nigra.
GPR14 is a high affinity receptor for urotensin-2 and urotensin-2B. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system. Referenced from Swiss-Prot Q9UKP6.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-F(1:100-500)
Flow-Cyt(2ug/Test)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Heart (Mouse) Lysate at 40 ug
Primary: Anti-GPR14 (bs-1806R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD

Blank control:U937.
Primary Antibody (green line): Rabbit Anti-GPR14 antibody (bs-1806R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells wereincubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.