Anti-MDC1 antibody
| 英文名称 | MDC1 |
| 中文名称 | DNA损伤关卡蛋白1抗体 |
| 别 名 | NFBD1; Nuclear factor with BRCT domains protein 1; DKFZp781A0122; Homologue to Drosophila photoreceptor protein calphotin; KIAA0170; MGC166888; MDC 1; Mediation of DNA damage checkpoint 1; Mediator of DNA damage checkpoint 1; Mediator of DNA Damage Checkpoint Protein 1; NFBD 1; NFBD1; Nuclear Factor with BRCT Domains Protein 1; MDC1_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:MDC1
IR:Immunogen Range:31-130/2089
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:9656
Swiss Prot:Q14676
Source:KLH conjugated synthetic peptide derived from human NFBD1:31-130/2089
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci. [provided by RefSeq].
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Protein:
embryo(mouse) lysates at 30ug;
testis(mouse) lysates at 30ug;
Primary: Anti-NFBD1/MDC1 (bs-2400R) at 1:200;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000;
ECL excitated the fluorescence;
Predicted band size : 188kD
Observed band size : 188kD
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MDC1) Polyclonal Antibody, Unconjugated (bs-2400R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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