Anti-IL-13Ra2 antibody (bs-2461R)

Anti-IL-13Ra2 antibody

DATASHEET

Host:Rabbit

Target Protein:IL-13Ra2

IR:Immunogen Range:81-180/380

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:3598

Swiss Prot:Q14627

Source:KLH conjugated synthetic peptide derived from human IL-13Ra2:81-180/380 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This protein is closely related to IL13RA1, a subunit of the interleukin 13 receptor complex. This protein binds IL13 with high affinity, but lacks cytoplasmic domain, and does not appear to function as a signal mediator. It is reported to play a role in the internalization of IL13.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
Flow-Cyt(1μg/Test)

Cross Reactive Species:Human
Mouse
Rat
Dog
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Raji(Human) Cell Lysate at 30 ug
HepG2(Human) Cell Lysate at 30 ug
Primary: Anti-IL-13Ra2 (bs-2461R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 41 kD

Blank control (Black line): Raji (Black).
Primary Antibody (green line): Rabbit Anti-IL-13Ra2 antibody (bs-2461R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA （10min）and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.