Anti-eIF4EBP1 antibody
| 英文名称 | eIF4EBP1 |
| 中文名称 | eIF4E结合蛋白抗体 |
| 别 名 | eIF4EBP1; Eukaryotic translation initiation factor 4E binding protein 1; Eukaryotic translation initiation factor 4E-binding protein 1; 4E BP1; 4EBP1; BP 1; BP1; eIF4E binding protein 1; eIF4E-binding protein 1; Eukaryotic translation initiation factor 4E binding protein 1; MGC4316; PHAS I; PHASI; PHAS-I; PHAS; 4E-BP1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; Phosphorylated heat and acid stable protein regulated by insulin 1; 4EBP1_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:eIF4EBP1
IR:Immunogen Range:65-118/118
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:1978
Swiss Prot:Q13541
Source:KLH conjugated synthetic peptide derived from human 4E-BP1:65-118/118
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008].
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Cow
Horse
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample: Ovary (Rat)Tissue Lysate at 30 ug
Primary: Anti- eIF4EBP1 (bs-2559R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 12kD
Observed band size: 12kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (eIF4EBP1) Polyclonal Antibody, Unconjugated (bs-2559R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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