Anti-CLEC2D antibody
| 英文名称 | CLEC2D |
| 中文名称 | CLEC2D蛋白抗体 |
| 别 名 | C-type lectin domain family 2 member D; C type lectin domain family 2 member D isoform 5; CLAX; LLT1; OCIL; Lectin like NK cell receptor; Lectin like transcript 1; Osteoclast inhibitory lectin; CLC2D_HUMAN; CLEC2D; CLEC2D protein; Lectin-like NK cell receptor; Lectin-like transcript 1; LLT-1; Osteoclast inhibitory lectin. |
DATASHEET
Host:Rabbit
Target Protein:CLEC2D
IR:Immunogen Range:101-207/207
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:29121
Swiss Prot:Q9UHP7
Source:KLH conjugated synthetic peptide derived from human CLEC2D:101-207/207
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:This gene encodes a member of the natural killer cell receptor C-type lectin family. The encoded protein inhibits osteoclast formation and contains a transmembrane domain near the N-terminus as well as the C-type lectin-like extracellular domain. Several alternatively spliced transcript variants have been identified, but the full-length nature of every transcript has not been defined.
Size:100ul
Concentration:1mg/ml
Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(1μg /test)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Horse
Rabbit
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Blank control (blue line): Raw264.7 (blue).
Primary Antibody (red line): Rabbit Anti-CLEC2D antibody (bs-2683R-FITC)in Overexpression hCLEC2D Raw264.7
Dilution: 1μg /10^6 cells
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed.
Tongji University/Professor Jia’s laboratory
Blank control:Mouse spleen.
Primary Antibody (green line): Rabbit Anti-CLEC2D antibody (bs-2683R-FITC)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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