Anti-phospho-FOXO4 (Ser262) antibody
| 英文名称 | phospho-FOXO4 (Ser262) |
| 中文名称 | 磷酸化叉头蛋白4抗体 |
| 别 名 | FOXO4 (phospho S262); p-FOXO4 (phospho S262); Fork head domain transcription factor AFX1; AFX; AFX1; Afxh; ALL1-fused gene from X chromosome; Fork head domain transcription factor AFX1; Forkhead box O4; Forkhead box protein O4; FOXO 4; Mixed lineage leukemia, translocated to, 7; MLLT7; Myeloid lymphoid or mixed lineage leukemia translocated to 7; Myeloid/lymphoid or mixed lineage leukemia, translocated to, 7; Putative fork head domain transcription factor AFX1. |
DATASHEET
Host:Rabbit
Target Protein:phospho-FOXO4 (Ser262)
IR:Immunogen Range:SS(p-S)NA
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:4303
Swiss Prot:P98177
Source:KLH conjugated Synthesised phosphopeptide derived from human FOXO4 around the phosphorylation site of Ser262:SS(p-S)NA
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:This gene encodes a member of the O class of winged helix/forkhead transcription factor family. Proteins encoded by this class are regulated by factors involved in growth and differentiation indicating they play a role in these processes. A translocation involving this gene on chromosome X and the homolog of the Drosophila trithorax gene, encoding a DNA binding protein, located on chromosome 11 is associated with leukemia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010]
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-phospho-FOXO4 (Ser262) (bs-3145R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD
Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-AFX Polyclonal Antibody, Unconjugated(bs-3145R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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