Anti-phospho-ATM (Ser1981) antibody
| 英文名称 | phospho-ATM (Ser1981) |
| 中文名称 | 磷酸化毛细血管扩张性共济失调症突变蛋白抗体 |
| 别 名 | ATM(Phospho-Ser1981); ATM (phospho S1981); AT complementation group A; AT complementation group C; AT complementation group D; AT complementation group E; AT mutated; AT protein;AT1;ATA;Ataxia telangiectasia gene mutated in human beings; Ataxia telangiectasia mutated; ATC; ATDC; ATE; ATM; Human phosphatidylinositol 3 kinase homolog; Serine protein kinase ATM; T cell prolymphocytic leukemia; TEL1; TPLL. |
DATASHEET
Host:Rabbit
Target Protein:phospho-ATM (Ser1981)
IR:Immunogen Range:EG(p-S)QS
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:472
Swiss Prot:Q13315
Source:KLH conjugated Synthesised phosphopeptide derived from human ATM around the phosphorylation site of Ser1981:EG(p-S)QS
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:ATM is a 370 kDa nuclear phosphoprotein involved in the autosomal recessive disease Ataxia Telangiectasia (AT). ATM belongs to a novel family of proteins associated with cell cycle regulation, apoptosis, and response to DNA damage repair (DNA damage caused by such things as ionizing irradiation activates ATM kinase). The C terminal region has extensive homology to the catalytic domains of Phosphatidylinositol 3 kinases (PI3 kinases).
Size:100ul
Concentration:1mg/ml
Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(1μg /test)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-ATM(Ser1981) Polyclonal Antibody, Unconjugated(bs-2272R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control (blue line): Hela (fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 20 min on ice).
Primary Antibody (green line): Rabbit Anti- phospho-ATM(Ser1981) antibody (bs-2272R),Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.
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