Anti-Phospho-CDK9 (Thr186) antibody
| 英文名称 | Phospho-CDK9 (Thr186) |
| 中文名称 | 磷酸化周期素依赖性激酶9抗体 |
| 别 名 | CCN T1; CCNT; CCNT 1; CCNT1; CDK9 associated C type protein; Cyc T1; Cyclin C related protein;cyclin T; cyclin T1; Cyclin T1b; Cyclin-T; cyclin-T1; ; CYCT 1; cycT1; HIVE1; Human immunodeficiency virus 1 expression; Human immunodeficiency virus type 1 (HIV 1) expression (elevated) 1; pTEFb subunit; Subunit of positive elongation transcription factor b; CDK9_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:Phospho-CDK9 (Thr186)
IR:Immunogen Range:RY(p-T)NR
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:1025
Swiss Prot:P50750
Source:KLH conjugated Synthesised phosphopeptide derived from human CDK9 around the phosphorylation site of Thr186:RY(p-T)NR
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin tightly associates with CDK9 kinase, and was found to be a major subunit of the transcription elongation factor p-TEFb. The kinase complex containing this cyclin and the elongation factor can interact with, and act as a cofactor of human immunodeficiency virus type 1 (HIV-1) Tat protein, and was shown to be both necessary and sufficient for full activation of viral transcription. This cyclin and its kinase partner were also found to be involved in the phosphorylation and regulation of the carboJNC-terminal domain (CTD) of the largest RNA polymerase II subunit.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample: Brain (Mouse) Lysate at 40 ug
Primary: Anti-p-CDK9 (Thr186) (bs-3097R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 48 kD
Tissue/cell: human laryngeal tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Phospho-CDK9 (Thr186) Polyclonal Antibody, Unconjugated(bs-3097R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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