Anti-Phospho-Dab1 (Ser491) antibody
| 英文名称 | Phospho-Dab1 (Ser491) |
| 中文名称 | 磷酸化Disabled 1抗体 |
| 别 名 | Dab1 (phospho Ser491); Dab1 (phospho S491); p-Dab1 (phospho Ser491); Phospho-Dab1 (Ser524)(human); Dab 1; Disabled homolog 1; Disabled homolog 1 Drosophila; Scm; Scr; Scrambler; Yot; Yotari; Dab, reelin signal transducer, homolog 1 (Drosophila); Dab1; DAB1_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:Phospho-Dab1 (Ser491)
IR:Immunogen Range:QS(p-S)PS
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:1600
Swiss Prot:O75553
Source:KLH conjugated Synthesised phosphopeptide derived from mouse Dab1 around the phosphorylation site of Ser491:QS(p-S)PS
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:The laminar organization of multiple neuronal types in the cerebral cortex is required for normal cognitive function. In mice, the disabled-1 gene plays a central role in brain development, directing the migration of cortical neurons past previously formed neurons to reach their proper layer. This gene is similar to disabled-1, and the protein encoded by this gene is thought to be a signal transducer that interacts with protein kinase pathways to regulate neuronal positioning in the developing brain. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined. [provided by RefSeq, Jul 2008]
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Cow
Horse
Rabbit
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample: 293T Cell (Human) Lysate at 40 ug
Primary: Anti-Phospho-Dab1 (Ser491)(bs-3116R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65kD
Observed band size: 63kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (P-Dab1 (Ser491)) Polyclonal Antibody, Unconjugated (bs-3116R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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