Anti-Phospho-DARPP32 (Thr75) antibody
| 英文名称 | Phospho-DARPP32 (Thr75) |
| 中文名称 | 磷酸化多巴胺/cAMP调节磷蛋白抗体 |
| 别 名 | DARPP32 (phospho T75); p-DARPP32 (phospho T75); PPP1R1B(phospho T75); DARPP 32; Dopamine and cAMP regulated neuronal phosphoprotein; Dopamine and cAMP regulated phosphoprotein DARPP 32; Dopamine and cAMP regulated phosphoprotein DARPP32; FLJ20940; IPPD; Neuronal phosphoprotein DARPP 32; PPP1R1B; Protein phosphatase 1 regulatory (inhibitor) subunit 1B; Protein phosphatase 1 regulatory subunit 1B; PPR1B_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:Phospho-DARPP32 (Thr75)
IR:Immunogen Range:AY(p-T)PP
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:84152
Swiss Prot:Q9UD71
Source:KLH conjugated Synthesised phosphopeptide derived from human DARPP32 around the phosphorylation site of Thr75:AY(p-T)PP
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:This gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011].
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Rabbit
Guinea Pig
,G.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Cerebral cortex (Mouse) Lysate at 40 ug
Primary: Anti- Phospho-DARPP32 (Thr75) (bs-3119R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 32 kD
Observed band size: 32 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-DARPP32 (Thr75)) Polyclonal Antibody, Unconjugated (bs-3119R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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