Anti-prox1 antibody (bs-2774R)

Anti-prox1 antibody

DATASHEET

Host:Rabbit

Target Protein:prox1

IR:Immunogen Range:151-250/737

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:5629

Swiss Prot:Q92786

Source:KLH conjugated synthetic peptide derived from human prox-1:151-250/737 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Prox1 is a prospero-related homeobox gene. Its expression pattern suggests it has a role in a variety of embryonic tissues and may play a fundamental role in early development of the CNS. It may regulate gene expression and the development of postmitotic undifferentiated young neurons.

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:2000-5000)
IHC-P(1:100-500)
Flow-Cyt(2ug/Test)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-prox1 Polyclonal Antibody, Unconjugated(bs-2774R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Blank control:K562.
Primary Antibody (green line): Rabbit Anti-prox1 antibody (bs-2774R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Blank control: HepG2.
Primary Antibody (green line): Rabbit Anti- antibody (bs-2774R-AF647)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.