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Anti-CD44v10 antibody (bs-2780R)

Anti-CD44v10 antibody (bs-2780R)

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Anti-CD44v10 antibody

英文名称CD44v10
中文名称CD44V10抗体
别    名LHR; BA-1; CD 44; CD44; CD44 antigen; CD44 molecule; CD44_HUMAN; CDw44; Cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; ECMR-III; Epican; Extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; hematopoietic cell E- and L-selectin ligand; Heparan sulfate proteoglycan; Hermes antigen; homing function and Indian blood group system; HSA; HUTCH-I; HUTCH1; Hyaluronate receptor; MDU2; MDU3; MIC4; MUTCH1; PGP-1; PGP1; Phagocytic glycoprotein 1; Phagocytic glycoprotein I.  

DATASHEET

Host:Rabbit

Target Protein:CD44v10

IR:Immunogen Range:218-227/742

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:960

Swiss Prot:P16070

Source:KLH conjugated synthetic peptide derived from human CD44v10:218-227/742 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq, Jul 2008].

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:2000-5000)
Flow-Cyt(1μg /Test)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Lane 1: Hela (Human) Cell Lysate at 30 ug
Lane 2: Huvec (Human) Cell Lysate at 30 ug
Lane 3: A549 (Human) Cell Lysate at 30 ug
Primary: Anti-CD44v10 (bs-2780R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 110-180 kD
Observed band size: 110-180 kD

Blank control(blue): Mouse spleen (fixed with 2% paraformaldehyde for 10 min at 37℃).
Primary Antibody:Rabbit Anti-CD44v10 antibody (bs-2780R,Green); Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions;
Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

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