Anti-Claudin 4 antibody (bs-2790R)

Anti-Claudin 4 antibody

DATASHEET

Host:Rabbit

Target Protein:Claudin 4

IR:Immunogen Range:21-120/209

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:1364

Swiss Prot:O14493

Source:KLH conjugated synthetic peptide derived from human CLDN4:21-120/209 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Claudin 1-8 proteins are a family of transmembrane proteins associated with tight junctions. Tight junctions are specialized regions of cell to cell contact made up of network of strands that act as a molecular gasket for preventing the leakage of ions, water etc. between cells. They are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Different tissues exhibit different Claudin composition.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:2000-5000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Rabbit
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample: Lung(Rat) lysate at 30ug;
Primary: Anti-Claudin-4 (bs-2790R) at 1:200 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution;
Predicted band size : 23kD
Observed band size : 23kD

Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Claudin-4 Polyclonal Antibody, Unconjugated(bs-2790R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining