Anti-CYP2C9 antibody (bs-2887R)

Anti-CYP2C9 antibody

DATASHEET

Host:Rabbit

Target Protein:CYP2C9

IR:Immunogen Range:351-450/490

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:1559

Swiss Prot:P11712

Source:KLH conjugated synthetic peptide derived from human CYP2C9:351-450/490 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooJNCgenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by rifampin. The enzyme is known to metabolize many xenobiotics, including phenytoin, tolbutamide, ibuprofen and S-warfarin. Studies identifying individuals who are poor metabolizers of phenytoin and tolbutamide suggest that this gene is polymorphic. The gene is located within a cluster of cytochrome P450 genes on chromosome 10q24. [provided by RefSeq, Jul 2008]

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:2000-5000)
IHC-P(1:200-800)
IHC-F(1:50-200)
IF(1:50-200)

Cross Reactive Species:Human
Mouse
Rat
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-CYP2C9 (bs-2887R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD

Paraformaldehyde-fixed, paraffin embedded (Mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CYP2C9) Polyclonal Antibody, Unconjugated (bs-2887R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.