Anti-Phospho-Lyn (Tyr397) antibody
| 英文名称 | Phospho-Lyn (Tyr397) |
| 中文名称 | 磷酸化膜相关蛋白酪氨酸激酶Lyn抗体 |
| 别 名 | ALyn (phospho Y397); Lyn (phospho Tyr397); p-Lyn (Tyr397); Hck 2; JTK 8; JTK8; ONCOGENE LYN; Tyrosine protein kinase LYN; V yes 1 Yamaguchi sarcoma viral related oncogene homolog; Yamaguchi sarcoma viral (v yes 1) related oncogene homolog; AA407514; EC 2.7.10.2; FLJ26625; LYN_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:Phospho-Lyn (Tyr397)
IR:Immunogen Range:NE(p-Y)TA
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:4067
Swiss Prot:P07948
Source:KLH conjugated synthesised phosphopeptide derived from human Lyn around the phosphorylation site of Tyr397:NE(p-Y)TA
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Lyn (also known as p53/56 Lyn) is a membrane associated protein tyrosine kinase (PTK) mostly expressed in hemopoietic cells which is important in cellular signaling. It contains an SH2 and SH3 domain and has been found to be cleaved after activation of caspases in apoptosis. A member of the Src family of PTKs, there are two known isoforms for Lyn which plays an indispensable role in the Fc epsilon RI (Fcer1) and the B cell IgM receptor signaling pathway and is essential for Syk activation and Lat phosphorylation after Fcer1 aggregation and can also phosphorylate Tec on multiple residues. Lyn can also be regulated by IL2 and IL3.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Pig
Rabbit
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Placenta (Mouse) Lysate at 40 ug
Lung (Mouse) Lysate at 40 ug
Primary: Anti-Phospho-Lyn (Tyr397)(bs-3257R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56kD
Observed band size: 63kD
Paraformaldehyde-fixed, paraffin embedded (mouse lung tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Lyn (Tyr397)) Polyclonal Antibody, Unconjugated (bs-3257R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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