Anti-Phospho-Met (Tyr1234 + Tyr1235) antibody
| 英文名称 | Phospho-Met (Tyr1234 + Tyr1235) |
| 中文名称 | 磷酸化肝细胞生长因子受体(原癌基因)抗体 |
| 别 名 | Met(Phospho-Tyr1234); Met (c-Met) (phospho Y1234 + Y1235);c-Met (phospho Y1234 + Y1235); AUTS9; c met; cmet; D249; Hepatocyte growth factor receptor; Hepatocyte growth factor receptor Precursor; HGF; HGF receptor; HGF SF receptor; HGF/SF receptor; HGFR; MET; Met proto oncogene tyrosine kinase; Met proto-oncogene (hepatocyte growth factor receptor); Met proto-oncogene; Met protooncogene; MET_HUMAN; Oncogene MET; Par4; Proto-oncogene c-Met; RCCP2; Renal cell carcinoma papillary 2 gene; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met. |
DATASHEET
Host:Rabbit
Target Protein:Phospho-Met (Tyr1234 + Tyr1235)
IR:Immunogen Range:KE(p-Y)(p-Y)SV
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:4233
Swiss Prot:P08581
Source:KLH conjugated synthesised phosphopeptide derived from human Met around the phosphorylation site of Tyr1234/1235:KE(p-Y)(p-Y)SV
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:c-Met, a member of the tyrosine kinase superfamily, is the receptor for hepatocyte growth factor, also known as scatter factor (HGF/SF). The mature c-Met protein is a disulfide-linked heterodimer with Mr=190 kDa composed of a heavily glycosylated alpha subunit that is completely extracellular in localization, and a beta subunit comprising an extracellular ligand binding domain, a single transmembrane domain, and a cytoplasmic tyrosine kinase domain. Cells expressing c-Met include epithelial cells, endothelial cells, blood cells of various types, and glomerular mesenchymal cells.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(1μg /Test)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Rabbit
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Tissue/cell: mouse stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Phospho-Met (Tyr1234+Tyr1235) Polyclonal Antibody, Unconjugated(bs-3272R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control (blue line): U251(fixed with 70% ethanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature).
Primary Antibody (green line): Rabbit Anti- Phospho-Met (Tyr1234 + Tyr1235) antibody (bs-3272R),Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.
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