Anti-MEK6 antibody (bs-2911R)

Anti-MEK6 antibody

DATASHEET

Host:Rabbit

Target Protein:MEK6

IR:Immunogen Range:251-334/334

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:5608

Swiss Prot:P52564

Source:KLH conjugated synthetic peptide derived from human MEK6:251-334/334 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Mitogen activated protein kinase kinase 6 (MEK6 or MKK6) belongs to the serine/threonine protein kinase family and the MAPK kinase subfamily (MAP2K, MKK or MEKs). MEK6, closely related to MEK3, catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38, thus activating it, in response to inflammatory cytokines and environmental stress.
As an essential component of p38 MAP kinase mediated signal transduction pathway, this protein is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
NIH/3T3(Mouse) Cell Lysate at 30 ug
Primary: Anti-MEK6 (bs-2911R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 35 kD

Sample:
A673(Human) Cell Lysate at 30 ug
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-MEK6 (bs-2911R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 35 kD