Anti-Phospho-MYL (Ser19) antibody
| 英文名称 | Phospho-MYL (Ser19) |
| 中文名称 | 磷酸化心脏肌球蛋白轻链2抗体 |
| 别 名 | Cardiac myosin light chain-2; Cardiac ventricular myosin light chain 2; CMH10; MLC 2v; MLC2; MYL 2; Myosin light chain 2 regulatory cardiac slow; Myosin light polypeptide 2 regulatory cardiac slow; Myosin regulatory light chain 2 ventricular cardiac muscle isoform; Myosin regulatory light chain 2; ventricular/cardiac muscle isoform; Regulatory light chain of myosin; RLC of myosin; Slow cardiac myosin regulatory light chain 2; MLRV_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:Phospho-MYL (Ser19)
IR:Immunogen Range:VF(p-S)MF
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:4633
Swiss Prot:P10916
Source:KLH conjugated Synthesised phosphopeptide derived from human MYL2 around the phosphorylation site of Ser19:VF(p-S)MF
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Rabbit
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Heart (Rat) Lysate at 40 ug
Primary: Anti-Phospho-MYL(Ser19) (bs-3295R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 18 kD
Observed band size: 18 kD
Tissue/cell: smooth muscle of mouse embryo; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Phospho-MYL(Ser19) Polyclonal Antibody, Unconjugated(bs-3295R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
好评度