Anti-ID2 antibody (bs-3515R)

Anti-ID2 antibody

DATASHEET

Host:Rabbit

Target Protein:ID2

IR:Immunogen Range:31-134/134

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:3398

Swiss Prot:Q02363

Source:KLH conjugated synthetic peptide derived from human GIG8/ID2:31-134/134 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The protein encoded by this gene belongs to the inhibitor of DNA binding (ID) family, and may play a role in negatively regulating cell differentiation. Members of the ID family are transcriptional regulators that contain a helix-loop-helix (HLH) domain but not a basic domain. They inhibit the functions of basic helix-loop-helix transcription factors in a dominant-negative manner, by suppressing their heterodimerization partners through the HLH domains.

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ID2) Polyclonal Antibody, Unconjugated (bs-3515R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ID2) Polyclonal Antibody, Unconjugated (bs-3515R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.