Anti-Phospho-PDGFRA (Tyr849) / PDGFRB (Tyr857) antibody
| 英文名称 | Phospho-PDGFRA (Tyr849) / PDGFRB (Tyr857) |
| 中文名称 | 磷酸化血小板源性生长因子受体α/β抗体 |
| 别 名 | Receptor beta (phospho Y857); p-Receptor beta (phospho Y857); PDGF Receptor alpha (phospho Y849); p-PDGF Receptor alpha (phospho Y849); PDGF receptor alpha + beta (phospho Y849 + Y857); PDGF receptor alpha + beta (phospho Tyr849 + Tyr857); PDGF Receptor alpha(Tyr849)/PDGF Receptor beta (Tyr857); Phospho-PDGFRA/PDGFRB(Tyr849/Tyr857); Platelet–dirived growth factor receptor-alpha; Alpha platelet derived growth factor receptor; CD 140a; CD140a; CD140a antigen; MGC74795; PDGF alpha chain; PDGF R alpha; PDGFR 2; PDGFR A; PDGFR alpha; PDGFR2; PDGFRA; Platelet derived growth factor receptor 2; Platelet derived growth factor receptor alpha; Platelet derived growth factor receptor alpha polypeptide; PGFRA_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:Phospho-PDGFRA (Tyr849) / PDGFRB (Tyr857)
IR:Immunogen Range:SN(p-Y)VS
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:5156
Swiss Prot:P16234
Source:KLH conjugated Synthesised phosphopeptide derived from human PDGF Receptor alpha around the phosphorylation site of Tyr849:SN(p-Y)VS
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:The PDGF Receptor Type A (Alpha platelet-derived growth factor receptor precursor, CD140a antigen), a 170kD protein, binds all three isoforms of PDGF with high affinity whereas the PDGF Receptor Type B, a 190kD protein, appears to bind only the PDGF BB homodimer with high affinity. Both receptors are transmembranous, ligand activated protein tyrosine kinases, which phosphorylate a number of important signal transduction proteins, which are bound with differential affinities via SH2 domains. The response of any given cell to PDGF will depend on the types of receptors displayed on the surface and isoforms of PDGF present in the extracellular environment.
Size:100ul
Concentration:1mg/ml
Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Cow
Horse
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PGFRA) Polyclonal Antibody, Unconjugated (bs-3320R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell:SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Antibody incubation with (Phospho-PDGFRA (Tyr849) / PDGFRB (Tyr857)) polyclonal Antibody, Unconjugated (bs-3320R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Blank control: Mouse kidney.
Primary Antibody (green line): Rabbit Anti-PDGFRA antibody (bs-3320R-AF488)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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