> 免疫组学 > 抗原和抗体 > 多克隆抗体 > Anti-phospho-PI3 kinase p85 alpha + gamma (Tyr467 + Tyr199) antibody (bs-3332R)Anti-phospho-PI3 kinase p85 alpha + gamma (Tyr467 + Tyr199) antibody
| 英文名称 | phospho-PI3 kinase p85 alpha + gamma (Tyr467 + Tyr199) |
| 中文名称 | 磷酸化磷脂酰肌醇激酶/PI3 Kinase P85α/γ抗体 |
| 别 名 | PI3 kinase p85 alpha (phospho Tyr467); PI3 kinase p55 gamma (phospho Tyr199); phospho-PI3 kinase p85 alpha / gamma (Tyr467 / Tyr199); PI3-kinase p85 subunit alpha; PI3-kinase p85 subunit gamma; GRB1; p50 alpha; p55 alpha; p85 alpha; p85; Phosphatidylinositol 3 kinase associated p85 alpha; Phosphatidylinositol 3 kinase regulatory 1; Phosphatidylinositol 3 kinase regulatory alpha subunit; Phosphoinositide 3 kinase regulatory subunit polypeptide 1 (p85 alpha); PI3 kinase p85 alpha subunit; PI3 kinase p85 subunit alpha; PI3K; PIK3R1; PtdIns 3 kinase p85 alpha; P55G_HUMAN; PtdIns 3 kinase p85 alpha; SH3_PI3K_p85alpha; P85A_HUMAN; PI 3-kinase p85α; PI 3-kinase p85 α; PI 3-kinase p85-α. |
DATASHEET
Host:Rabbit
Target Protein:phospho-PI3 kinase p85 alpha + gamma (Tyr467 + Tyr199)
IR:Immunogen Range:RL(p-Y)EE
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:5295
Swiss Prot:P27986
Source:KLH conjugated Synthesised phosphopeptide derived from human PI3 kinase p85 alpha around the phosphorylation site of p85 Tyr467:RL(p-Y)EE
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that generates phosphatidylinositol 3, 4, 5-triphosphate in response to receptor activation in many signal transduction pathways. Class IA PI3Ks exist as a heterodimer of a catalytic 110 kDa (p110) and a regulatory p85 subunit (e.g. p85 alpha). p85 alpha is an adaptor molecule that regulates the activity of the catalytic p110 subunit by binding to phosphorylated receptor tyrosine kinases (RTKs) through its SH2 domain and mediating the interaction between p110 and the plasma membrane. p85 alpha is necessary for insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(1ug/test)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Rabbit
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-PI3 kinase p85 alpha+gamma (Tyr467+Tyr199) Polyclonal Antibody, Unconjugated(bs-3332R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: Molt4.
Primary Antibody (green line): Rabbit Anti- phospho-PI3 kinase p85 alpha + gamma (Tyr467 + Tyr199)/FITC Conjugated antibody (bs-3332R-FITC)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-FITC .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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