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Anti-Phospho-PLCG 2 (Tyr1217) antibody (bs-3338R)

Anti-Phospho-PLCG 2 (Tyr1217) antibody (bs-3338R)

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Anti-Phospho-PLCG 2 (Tyr1217) antibody

英文名称Phospho-PLCG 2 (Tyr1217)
中文名称磷酸化磷酯酶Cγ2抗体
别    名PLCG 2 (phospho Y1217); p-PLCG 2 (phospho Y1217); PLCG2(phospho Y1217); Phospho-PLC γ2(Tyr1217); Phospho-PLC gamma 2/PLCG2(Tyr1217); PLC 2; PLC gamma 2; PLC IV; PLCG2; 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 2; EC 3.1.4.11; Phosphoinositide phospholipase C; Phospholipase C gamma 2; Phospholipase C, gamma 2 (phosphatidylinositol specific); PLCG2_HUMAN.   

DATASHEET

Host:Rabbit

Target Protein:Phospho-PLCG 2 (Tyr1217)

IR:Immunogen Range:FL(p-Y)DT

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:5336

Swiss Prot:P16885

Source:KLH conjugated Synthesised phosphopeptide derived from human PLC gamma 2 around the phosphorylation site of Tyr1217:FL(p-Y)DT 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Enzymes of the phospholipase C family catalyze the hydrolysis of phospholipids to yield diacylglycerols and water soluble phosphorylated derivatives of the lipid head groups. A number of these enzymes have specificity for phosphoinositides. Of the phosphoinositide specific phospholipase C enzymes, C beta is regulated by heterotrimeric G protein coupled receptors, while the closely related C gamma 1 and C gamma 2 enzymes are controlled by receptor tyrosine kinases. The C gamma 1 and C gamma 2 enzymes are composed of phospholipase domains that flank regions of homology to noncatalytic domains of the SRC oncogene product, SH2 and SH3.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(1μg /Test)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Rabbit
Guinea Pig
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Blank control(blue): Hep G2 Cells(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice) . Primary Antibody: Rabbit Anti-Phospho-PLCG 2 (Tyr1217)/AF647 Conjugated antibody (bs-3338R/AF647), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.

Blank control(blue): Hep G2 Cells(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice) .
Primary Antibody: Rabbit Anti-Phospho-PLCG 2 (Tyr1217)/AF647 Conjugated antibody (bs-3338R/AF647), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.

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