Anti-Phospho-PLK1 (Ser137) antibody
| 英文名称 | Phospho-PLK1 (Ser137) |
| 中文名称 | 磷酸化丝/苏氨酸蛋白激酶Plk1抗体 |
| 别 名 | PLK1 (phospho S137); p-PLK1 (phospho S137); PLK1 (Phospho-Thr137); PLK1 (Phospho Thr137); p-PLK1 (Thr137); p-PLK1 (T137); Polo-Like Kinase(phospho T137); PLK 1; PLK; polio like kinase; Polo like kinase 1; Polo-like kinase 1; Serine/threonine protein kinase 13; Serine/threonine protein kinase PLK1; Serine/threonine-protein kinase; STPK 13; STPK13; Polo like kinase kinase; Cell cycle regulated protein kinase; PLK-1; plk1; PLK1_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:Phospho-PLK1 (Ser137)
IR:Immunogen Range:R(p-S)LL
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:5347
Swiss Prot:P53350
Source:KLH conjugated Synthesised phosphopeptide derived from human PLK1 around the phosphorylation site of Ser137:R(p-S)LL
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:PLK1 (polo-like kinase 1) is a member of the serine/threonine protein kinase family, cdc5/polo subfamily. PLK1 contains two polo box domains with a predicted molecular weight of 68 kDa. PLK1 has been shown to regulate cdc2/cyclin B through phosphorylation and activation of cdc25c phosphatase. PLK1 is modified by phosphorylation at Threonine 210. PLK1 may also be required for cell division. Depletion of PLK1 results in apoptosis and deregulation of expression of PKL1 is correlated with development of many malignancies.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-1000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Rabbit
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Large intestine (Mouse) Lysate at 40 ug
Primary: Anti-Phospho-PLK1 (Ser137) (bs-3345R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 68 kD
Observed band size: 68 kD
Paraformaldehyde-fixed, paraffin embedded (rat colon tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PLK1 (Ser137)) Polyclonal Antibody, Unconjugated (bs-3345R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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