Anti-Phospho-eNOS (Ser1177) antibody
| 英文名称 | Phospho-eNOS (Ser1177) |
| 中文名称 | 磷酸化一氧化氮合成酶3（内皮型）抗体 |
| 别 名 | eNOS (phospho S1177); eNOS(phospho S1177); p-eNOS (phospho S1177); eNOS (Phospho-Ser1177); cNOS; Constitutive NOS; EC NOS; ecNOS; Endothelial nitric oxidase synthase; Endothelial nitric oxide synthase; Endothelial nitric oxide synthase 3; Endothelial NOS; Nitric oxide synthase 3 (endothelial cell); Nitric oxide synthase 3; Nitric oxide synthase 3 endothelial cell; Nitric oxide synthase endothelial; nitric oxide synthase, endothelial; NOS 3; NOS III; NOS type III; NOS3; NOSIII; NOS3_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:Phospho-eNOS (Ser1177)
IR:Immunogen Range:TQ(p-S)FS
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:4846
Swiss Prot:P29474
Source:KLH conjugated Synthesised phosphopeptide derived from human eNOS around the phosphorylation site of Ser1177:TQ(p-S)FS
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:eNOS is a calcium/calmodulin dependent enzyme which undergoes several post translational modifications, including acylation with myristate and palmitate, and phosphorylation on numerous residues. As with the other members of the NOS family, eNOS derives the diffusible multifunctional second messenger NO from L arginine through a series of reactions in which L citrulline is an intermediate. eNOS plays an important role in controlling vascular tone, platelet aggregation, and cardiac myocyte function.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(2μg /Test)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Rabbit
Sheep
Guinea Pig
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Embryo (Mouse) Lysate at 40 ug
Primary: Anti- p-eNOS (Ser1177) (bs-3447R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 133 kD
Observed band size: 133 kD
The blue histogram is unstained cells(HepG 2 cells). The Orange histogram is cells stained with Rabbit IgG/FITC (bs-0295P-FITC) The green histogram is cells stained with Rabbit Anti-Phospho-eNOS (Ser1177)/FITC Conjugated antibody (bs-3447R-FITC). Isotype control: Cell lines treated with Rabbit IgG/FITC (bs-0295P-FITC) instead of the primary antibody to confirm that primary antibody binding is specific. 2μg in 100 μl 1 X PBS containing 0.5% BSA.
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