Anti-Phospho-RPS6KA1 (Thr359 + Ser363) antibody
| 英文名称 | Phospho-RPS6KA1 (Thr359 + Ser363) |
| 中文名称 | 磷酸化丝氨酸/苏氨酸激酶p90RSK蛋白抗体 |
| 别 名 | RSK1 p90 (phospho T359 + S363); p-RSK1 p90 (phospho T359 + S363); p90 RSK1; p90rsk; p90RSK; p90S6K; pp90RSK1; Ribosomal protein S6 kinase 90kD 1; Ribosomal protein S6 kinase 90kD polypeptide 1; Ribosomal protein S6 kinase 90kDa polypeptide 1; Ribosomal protein S6 kinase alpha 1; Ribosomal protein S6 kinase polypeptide 1; Ribosomal S6 kinase 1; RPS6K1 alpha; rps6ka; Rps6ka1; RSK 1; RSK 1 p90; RSK; RSK1; S6K alpha 1; 90 kDa ribosomal protein S6 kinase 1; dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1; dJ590P13.1; EC 2.7.11.1; HU 1; HU1; MAP kinase activated protein kinase 1a; MAPKAP kinase 1A; MAPKAPK1A; MGC79981; Mitogen-activated protein kinase-activated protein kinase 1A; OTTHUMP00000004113. |
DATASHEET
Host:Rabbit
Target Protein:Phospho-RPS6KA1 (Thr359 + Ser363)
IR:Immunogen Range:SR(p-T)PKD(p-S)PG
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:6195
Swiss Prot:Q15418
Source:KLH conjugated Synthesised phosphopeptide derived from human p90RSK around the phosphorylation site of Thr359/Ser363:SR(p-T)PKD(p-S)PG
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Rsk1 is a member of a family of 90kDa ribosomal protein S6 kinases, which includes Rsk1, Rsk2 and Rsk3. These are broadly expressed serine/threonine protein kinases activated in response to mitogenic stimuli, including extracellular signal regulated protein kinases Erk1 and Erk2. Rsk1 is activated by MAPK in vitro and in vivo via phosphorylation. Active Rsks appear to play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c Fos and CREB.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Bone (Mouse) Lysate at 40 ug
Primary: Anti-Phospho-RPS6KA1 (Thr359 + Ser363) (bs- 3363R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 81 kD
Observed band size: 81 kD
Tissue/cell: Rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Phospho-RPS6KA1 (Thr359 + Ser363) Polyclonal Antibody, Unconjugated(bs-3363R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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