Anti-TIF1 gamma antibody
| 英文名称 | TIF1 gamma |
| 中文名称 | 转录中介因子Tif1γ抗体 |
| 别 名 | Trim33; RFG7; Rfg7 protein; TF1G; TIF1-gamma; TIF1G; TIF 1G; TIF1GAMMA; TIFGAMMA; Transcription intermediary factor 1-gamma; Transcriptional intermediary factor 1 gamma; Trim 33; Tripartite motif containing 33; Tripartite motif containing 33 protein; tripartite motif-containing 33; Tripartite motif-containing protein 33; E3 ubiquitin-protein ligase TRIM33; Ectodermin homolog; Protein Rfg7; PTC7 antibody; Ret fused gene 7; RET-fused gene 7 protein; RFG7; KIAA1113; TRI33_HUMAN; Ectodermin homolog; RET-fused gene 7 protein; Protein Rfg7; Transcription intermediary factor 1-gamma. |
DATASHEET
Host:Rabbit
Target Protein:TIF1 gamma
IR:Immunogen Range:981-1127/1127
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:51592
Swiss Prot:Q9UPN9
Source:KLH conjugated synthetic peptide derived from human TIF1 gamma:981-1127/1127
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:TIF1 gamma is a member of the Transcriptional Intermediary Factor 1 family, which serve as coactivators and corepressors. Its structure is similar to TIF1 alpha and TIF1 beta, exhibiting multiple domains (RING finger, coiled coil, B boxes, PHD/TTC, and bromodomain). TIF1 gamma functions in cell differentiation and development, playing a role in differentiation of hematopoietic cells.
Size:100ul
Concentration:1mg/ml
Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TIF1 gamma ) Polyclonal Antibody, Unconjugated (bs-3582R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Tissue/cell: mouse intestine tissue; Frozen section;
Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TIF1 gamma Polyclonal Antibody, Unconjugated(bs-3582R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: Mouse kidney.
Primary Antibody (green line): Rabbit Anti-RFG7 antibody (bs-3582R-A488)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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