Anti-Phospho-Stat2 (Tyr690) antibody
| 英文名称 | Phospho-Stat2 (Tyr690) |
| 中文名称 | 磷酸化信号转导和转录激活因子2抗体 |
| 别 名 | Stat2 (phospho-Tyr690); Stat2 (phospho Y690); Stat2 (phospho Tyr690); p-Stat2 (Tyr690); signal transducers and activators of transduction2; Homo sapiens interferon alpha induced transcriptional activator; interferon alpha induced transcriptional activator; ISGF3; P113; signal transducer and activator of transcription 2 113kD; STAT113; STAT2_HUMAN; p113. |
DATASHEET
Host:Rabbit
Target Protein:Phospho-Stat2 (Tyr690)
IR:Immunogen Range:RK(p-Y)LK
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:6773
Swiss Prot:P52630
Source:KLH conjugated Synthesised phosphopeptide derived from human Stat2 around the phosphorylation site of Tyr690:RK(p-Y)LK
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. In response to interferon (IFN), this protein forms a complex with STAT1 and IFN regulatory factor family protein p48 (ISGF3G), in which this protein acts as a transactivator, but lacks the ability to bind DNA directly. Transcription adaptor P300/CBP (EP300/CREBBP) has been shown to interact specifically with this protein, which is thought to be involved in the process of blocking IFN-alpha response by adenovirus. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010].
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Horse
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:Brain (Mouse) Lysate at 40 ug
Primary: Anti-Phospho-Stat2 (Tyr690)(bs-3428R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 94kD
Observed band size: 105kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Stat2 (Tyr690)) Polyclonal Antibody, Unconjugated (bs-3428R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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