Anti-Phospho-Syk (Tyr525 + Tyr526) antibody (bs-3434R)

Anti-Phospho-Syk (Tyr525 + Tyr526) antibody

DATASHEET

Host:Rabbit

Target Protein:Phospho-Syk (Tyr525 + Tyr526)

IR:Immunogen Range:EN(p-Y)(p-Y)KA

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:6850

Swiss Prot:P43405

Source:KLH conjugated Synthesised phosphopeptide derived from human Syk around the phosphorylation site of Tyr525/526:EN(p-Y)(p-Y)KA 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Syk (72 kDa) is a non receptor protein tyrosine kinase that plays an important role in immune receptor signal transduction and is implicated in endothelial cell functions, including cell growth and migration. SYK is a positive effector of BCR stimulated responses. It couples the B cell antigen receptor (BCR) to the mobilization of calcium ions either through a phosphoinositide 3 kinase dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C gamma dependent pathway, when phosphorylated on Tyr 342 and Tyr 346. Thus the differential phosphorylation of SYK can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ions. Alternate Names: p72syk; Spleen tyrosine kinase; Tyrosine protein kinase SYK.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Rabbit
Guinea Pig
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Lane1: Embryo (Mouse) Lysate at 30 ug
Lane2: Thymus (Mouse) Cell Lysate at 30 ug
Primary: Anti- phospho-Syk (Tyr525/526) (bs-3434R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size : 72kD
Observed band size : 70kD

Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Syk (Tyr525 + Tyr526)) Polyclonal Antibody, Unconjugated (bs-3434R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.