Anti-phospho-VEGFR2 (Tyr1175) antibody
| 英文名称 | phospho-VEGFR2 (Tyr1175) |
| 中文名称 | 磷酸化血管内皮生长因子受体2抗体 |
| 别 名 | VEGFR2(Phospho-Tyr1175); VEGFR2(Phospho-Y1175); p-VEGFR2(Tyr1175); p-VEGFR2(Y1175); CD309; CD309 antigen; Fetal liver kinase 1; FLK-1; FLK1; KDR; Kinase insert domain receptor (a type III receptor tyrosine kinase); Kinase insert domain receptor; KRD1; Ly73; Protein tyrosine kinase receptor FLK1; Protein-tyrosine kinase receptor flk-1; Tyrosine kinase growth factor receptor; Vascular endothelial growth factor receptor 2; VEGFR 2; VEGFR; VEGFR-2; VEGFR2; VGFR2_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:phospho-VEGFR2 (Tyr1175)
IR:Immunogen Range:KD(p-Y)IV
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:3791
Swiss Prot:P35968
Source:KLH conjugated Synthesised phosphopeptide derived from human VEGFR2 around the phosphorylation site of Tyr1175:KD(p-Y)IV
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq, May 2009].
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
Flow-Cyt(2ug/test)
Cross Reactive Species:Mouse
Rat
Dog
Pig
Cow
Horse
Rabbit
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Lane 1: Placenta (Mouse) Lysate at 40 ug
Lane 2: Adrenal gland (Rat) Lysate at 40 ug
Primary: Anti-phospho-VEGFR2 (Tyr1175) (bs-3468R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 230 kD
Observed band size: 220 kD
Blank control:Huvec.
Primary Antibody (green line): Rabbit Anti-phospho-VEGFR2 (Tyr1175) antibody (bs-3468R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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