Anti-FPRL1 antibody (bs-3654R)

Anti-FPRL1 antibody

DATASHEET

Host:Rabbit

Target Protein:FPRL1

IR:Immunogen Range:51-150/351

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:2358

Swiss Prot:P25090

Source:KLH conjugated synthetic peptide derived from human FPRL1:51-150/351 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:FPRL1 is a low affinity receptor to N-formyl-methionyl peptides, which are powerful neutrophils chemotactic factors. Binding of FMLP to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The activation of LXA4R could result in an anti-inflammatory outcome counteracting the actions of proinflammatory signals such as LTB4 (leukotriene B4). FPRL1 has been reported in phagocytes, monocytes, neutrophils, differentiated myeloid cells from bone marrow, granulocyte HL-60 cells, and synovial fibroblasts. ESTs have been isolated from blood, leukocyte, lung, and placenta libraries.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)

Cross Reactive Species:Human
Mouse
Rat
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:Liver(Mouse) Lysate at 40 ug
Primary: Anti-FPRL1(bs-3654R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kD
Observed band size: 47 kD

Sample:
kidney (Mouse) Lysate at 40 ug
Primary: Anti-FPRL1 (Bs-3654R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kD
Observed band size: 48 kD

Blank control: Mouse spleen.
Primary Antibody (green line): Rabbit Anti-FPRL1 antibody (bs-3654R-FITC)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-APC
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.Acquisition of 20,000 events was performed.

Blank control: Mouse spleen.
Primary Antibody (green line): Rabbit Anti-FPRL1 antibody (bs-3654R-FITC)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.

Blank control (Black line): U937(Black).
Primary Antibody (green line): Rabbit Anti-FPRL1/AF488 antibody (bs-3654R-AF488)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-AF488 .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.