Anti-TNFSF15 antibody
| 英文名称 | TNFSF15 |
| 中文名称 | 肿瘤坏死因子配体超家族成员15抗体 |
| 别 名 | TL1A; TL1 A; TL 1A; TL-1A; tl1; tl1a; tnf ligand related molecule 1; TNF ligand-related molecule 1; tnf superfamily ligand tl1a; TNF15_HUMAN; Tumor necrosis factor ligand superfamily member 15; Vascular endothelial cell growth inhibitor; Vascular endothelial growth inhibitor 192a; Vascular endothelial growth inhibitor; vegi; VEGI192A antibodysecreted form; MGC129934; MGC129935. |
DATASHEET
Host:Rabbit
Target Protein:TNFSF15
IR:Immunogen Range:161-251/251
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:9966
Swiss Prot:O95150
Source:KLH conjugated synthetic peptide derived from human TNFSF15:161-251/251
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Receptor for TNFRSF25 and TNFRSF6B. Mediates activation of NF-kappa-B. Inhibits vascular endothelial growth and angiogenesis (in vitro). Promotes activation of caspases and apoptosis.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Rabbit
Guinea Pig
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
bone(mouse) Lysate at 40 ug
Primary: Anti-TNFSF15 (bs-5092R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28kD
Observed band size: 38 kD
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TNFSF15 Polyclonal Antibody, Unconjugated(bs-5092R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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