Anti-CHRDL2 antibody (bs-3860R)

Anti-CHRDL2 antibody 

DATASHEET

Host:Rabbit

Target Protein:CHRDL2

IR:Immunogen Range:351-429/429

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:25884

Swiss Prot:Q6WN34

Source:KLH conjugated synthetic peptide derived from human CHRDL2:351-429/429 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:CHRDL2 is a novel chordin like protein that can act as a BMP antagonist. A member of the chordin family of proteins, it contains a signal peptide and three CR (cysteine-rich repeat) domains. When expressed as a recombinant protein it is secreted and binds to activin A, but not to BMP-2, -4, -6. Differential expression has been detected in developing chondrocytes, myoblasts, osteoblasts, and osteoarthritic joints. Complex alternative splicing of CHRDL2 potentially results in distinct isoforms that differ at their C termini, in the expression of signal peptide, and in the content of CR domains. CHRDL2 was originally characterized as a novel protein exclusively expressed in breast, lung, and colon tumors.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Cow
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Uterus (Rat) Lysate at 40 ug
Testis (Rat) Lysate at 40 ug
Primary: Anti- CHRDL2 (bs-3860R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 45 kD

Sample:
Uterus(Mouse) Lysate at 40 ug
Primary: Anti- CHRDL2 (bs-3860R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 45 kD