Anti-NPRC antibody (bs-2333R)

Anti-NPRC antibody 

DATASHEET

Host:Rabbit

Target Protein:NPRC

IR:Immunogen Range:301-400/541

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:4883

Swiss Prot:P17342

Source:KLH conjugated synthetic peptide derived from human NPR-C:301-400/541 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Natriuretic peptide receptor C does not exhibit guanylate cyclase activity. There seem to be at least three ANP receptors: two with guanylate cyclase activity (ANPA and ANPB) and one (ANPC) which is probably responsible for the clearance of ANP from the circulation without a role in signal transduction.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Cow
Horse
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
HL-60 (Human) Cell Lysate at 40 ug
A431 (Human) Cell Lysate at 40 ug
Kidney (Mouse) Lysate at 40 ug
Primary: Anti-NPRC (bs-2333R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kD
Observed band size: 57 kD

Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NPRC) Polyclonal Antibody, Unconjugated (bs-2333R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.