Anti-phospho-C-Myc (Thr358) antibody
| 英文名称 | phospho-C-Myc (Thr358) |
| 中文名称 | 磷酸化致癌基因C-Myc抗体 |
| 别 名 | Myc(Phospho-Thr358); Myc(Phospho-T358); p-Myc(Thr358); p-Myc(T358); AU016757; Avian myelocytomatosis viral oncogene homolog; bHLHe39; c Myc; Cellular myelocytomatosis oncogene; MGC105490; MRTL; Myc protein; Myc proto oncogene protein; Myc-related translation/localization regulatory factor; Myc2; myca; Myelocytomatosis oncogene a; Myelocytomatosis oncogene; Niard; Nird; Oncogene Myc; Protooncogene homologous to myelocytomatosis virus; RNCMYC; Transcription factor p64; Transcriptional regulator Myc-A; v myc avian myelocytomatosis viral oncogene homolog; v myc myelocytomatosis viral oncogene homolog (avian); V-Myc avian myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene homolog (avian); zc-myc; MYC_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:phospho-C-Myc (Thr358)
IR:Immunogen Range:RR(p-T)HN
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:4609
Swiss Prot:P01106
Source:KLH conjugated Synthesised phosphopeptide derived from human C-Myc around the phosphorylation site of Thr358:RR(p-T)HN
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq, Jul 2008].
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
Flow-Cyt(1μg/Test)
Cross Reactive Species:Human
Horse
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
A431(Human) Lysate at 30 ug
U251(Human) Lysate at 30 ug
Huvec(Human) Lysate at 30 ug
Raji(Human) Lysate at 30 ug
U937(Human) Lysate at 30 ug
Primary: Anti- phospho-C-Myc(Thr358) (bs-4017R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kD
Observed band size: 62 kD
Blank control (blue line): Hela cells (fixed with 70% methanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃).
Primary Antibody (green line): Rabbit Anti-phospho-C-Myc(Thr358) antibody (bs-4017R)，Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE，Dilution: 1μg /test.
好评度