Anti-ENO1 antibody
| 英文名称 | ENO1 |
| 中文名称 | MYC启动子结合蛋白1抗体 |
| 别 名 | 2 phospho D glycerate hydro lyase; Alpha enolase 1; Alpha enolase; C myc promoter binding protein; EC 4.2.1.11; ENO1L1; Enolase 1 (alpha); Enolase 1 (alpha) like 1; Enolase 1; MBP 1; MBP1; MBPB1; MPB 1; MPB1; MYC promoter binding protein 1; NNE; Non neural enolase; Phosphopyruvate hydratase; Plasminogen binding protein; PPH; Non Neuronal Enolase; ENOA_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:ENO1
IR:Immunogen Range:51-150/434
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:2023
Swiss Prot:P06733
Source:KLH conjugated synthetic peptide derived from human ENO1:51-150/434
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:ENO1 encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Cow
Rabbit
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Embryo (Mouse) Lysate at 40 ug
A673(Human) Cell Lysate at 30 ug
NIH/3T3(Mouse) Cell Lysate at 30 ug
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-ENO1 (bs-3978R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 48 kD
Tissue/cell: Rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ENO1 Polyclonal Antibody, Unconjugated(bs-3978R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
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