Anti-Glucose 6 phosphatase alpha antibody (bs-4044R)

Anti-Glucose 6 phosphatase alpha antibody

DATASHEET

Host:Rabbit

Target Protein:Glucose 6 phosphatase alpha

IR:Immunogen Range:81-180/357

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:2538

Swiss Prot:P35575

Source:KLH conjugated synthetic peptide derived from human Glucose 6 phosphatase alpha:81-180/357 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Glucose-6-phosphatase (G6Pase) is a multi-subunit integral membrane protein of the endoplasmic reticulum that is composed of a catalytic subunit and transporters for G6P, inorganic phosphate, and glucose. This gene (G6PC) is one of the three glucose-6-phosphatase catalytic-subunit-encoding genes in human: G6PC, G6PC2 and G6PC3. Glucose-6-phosphatase catalyzes the hydrolysis of D-glucose 6-phosphate to D-glucose and orthophosphate and is a key enzyme in glucose homeostasis, functioning in gluconeogenesis and glycogenolysis. Mutations in this gene cause glycogen storage disease type I (GSD1). This disease, also known as von Gierke disease, is a metabolic disorder characterized by severe hypoglycemia associated with the accumulation of glycogen and fat in the liver and kidneys.[provided by RefSeq, Feb 2011]

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
Flow-Cyt(0.2ug/test)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Rabbit
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

U-937 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with bs-4044R Antibody at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

U-937 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with bs-4044R Antibody at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).