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Anti-AKR1D1 antibody (bs-5026R)

Anti-AKR1D1 antibody (bs-5026R)

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Anti-AKR1D1 antibody 

英文名称AKR1D1
中文名称醛固酮还原酶家族1成员D1抗体
别    名3-oxo-5-beta-steroid 4-dehydrogenase; 3o5bred; AK1D1_HUMAN; AKR1D1; aldo keto reductase family 1 member D1 (delta 4 3 ketosteroid 5 beta reductase); Aldo keto reductase family 1 member D1; Aldo-keto reductase family 1 member D1; CBAS2; Delta(4) 3 ketosteroid 5 beta reductase; Delta(4) 3 oxosteroid 5 beta reductase; Delta(4)-3-ketosteroid 5-beta-reductase; Delta(4)-3-oxosteroid 5-beta-reductase; SRD5B1; steroid 5 beta reductase beta polypeptide 1 (3 oxo 5 beta steroid delta 4 dehydrogenase beta 1); steroid 5 beta reductase.  

DATASHEET

Host:Rabbit

Target Protein:AKR1D1

IR:Immunogen Range:101-200/326

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:6718

Swiss Prot:P51857

Source:KLH conjugated synthetic peptide derived from human AKR1D1:101-200/326 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Efficiently catalyzes the reduction of progesterone, androstenedione, 17-alpha-hydroJNCprogesterone and testosterone to 5-beta-reduced metabolites. The bile acid intermediates 7-alpha,12-alpha-dihydroJNC-4-cholesten-3-one and 7-alpha-hydroJNC-4-cholesten-3-one can also act as substrates.

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Cow
Horse
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AKR1D1) Polyclonal Antibody, Unconjugated (bs-5026R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AKR1D1) Polyclonal Antibody, Unconjugated (bs-5026R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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