Anti-CROT antibody (bs-5048R)

Anti-CROT antibody

DATASHEET

Host:Rabbit

Target Protein:CROT

IR:Immunogen Range:41-140/612

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:54677

Swiss Prot:Q9UKG9

Source:KLH conjugated synthetic peptide derived from human CROT:41-140/612 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:CROT (Carnitine O-octanoyltransferase) is involved in the beta-oxidation of fatty acids. It catalyzes the reversible transfer of fatty acyl groups between CoA and carnitine. It has highest activity towards the C6 to C10 chain length substrate. It is a crucial step in the transport of medium- and long-chain acyl-CoA out of the mammalian peroxisome to the cytosol and mitochondria.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Horse
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample: 293T Cell (Human) Lysate at 40 ug
Primary: Anti-CROT (bs-5048R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 70 kD
Observed band size: 70 kD

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CROT Polyclonal Antibody, Unconjugated(bs-5048R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining