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Anti-ME1 antibody (bs-5084R)

Anti-ME1 antibody (bs-5084R)

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Anti-ME1 antibody

英文名称ME1
中文名称胞浆苹果酸酶1抗体
别    名Cytosolic malic enzyme 1; HUMNDME; Malate dehydrogenase; Malic enzyme 1; Malic enzyme 1, NADP(+) dependent, cytosolic; Malic enzyme 1, soluble; Malic enzyme, cytoplasmic; MES; NADP dependent malic enzyme; NADP ME; Pyruvic malic carboJNClase.Cytosolic malic enzyme 1; HUMNDME; Malate dehydrogenase; Malic enzyme 1; Malic enzyme 1, NADP(+) dependent, cytosolic; Malic enzyme 1, soluble; Malic enzyme, cytoplasmic; MES; NADP dependent malic enzyme; NADP ME; Pyruvic malic carboJNClase.  

DATASHEET

Host:Rabbit

Target Protein:ME1

IR:Immunogen Range:451-550/572

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:4199

Swiss Prot:P48163

Source:KLH conjugated synthetic peptide derived from human NADP ME:451-550/572 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:ME1 is a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboJNClation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Rabbit
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Hela(human)cell Lysate at 30 ug
Primary: Anti- ME1 (bs-5084R)at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 64kD
Observed band size: 64kD

Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ME1) Polyclonal Antibody, Unconjugated (bs-5084R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Anti-ME1 antibody (bs-5084R)

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