Anti-AKT3 antibody (bs-5146R)

Anti-AKT3 antibody

DATASHEET

Host:Rabbit

Target Protein:AKT3

IR:Immunogen Range:101-200/479

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:10000

Swiss Prot:Q9Y243

Source:KLH conjugated synthetic peptide derived from human AKT3:101-200/479 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Akt, protein kinase B (PKB), is a serine/threonine kinase which is involved in many cellular signaling pathways and acts as a transducer of many functions initiated by growth factor receptors that activate phosphtidylinositol 3 kinase (PI 3 kinase). The major activity of Akt/PKB is to mediate cell survival. Akt/PKB is also believed to be a critical factor in the genesis of cancer as the tumor suppressor PTEN was found to antagonise PI3 kinase and Akt/PKB kinase activity. There are 3 known isoforms of this enzyme in mammalian cells (1/alpha, 2/beta and 3/gamma). IGF1 leads to the activation of AKT3, which may play a role in regulating cell survival. It is capable of phosphorylating several known proteins.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:200-600)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Horse
Rabbit
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Cerebrum(Mouse)Lysate at 40 ug
Testis (Mouse) Lysate at 40 ug
Primary: Anti- AKT3 (bs-5146R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 60 kD

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AKT3) Polyclonal Antibody, Unconjugated (bs-5146R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.