Anti-phospho-APC1 (Ser355) antibody
| 英文名称 | phospho-APC1 (Ser355) |
| 中文名称 | 磷酸化细胞周期末期促进复合蛋白APC1抗体 |
| 别 名 | Apc1 (phospho S355); phospho-ANAPC1 (Ser355); ANAPC 1; ANAPC1; Anaphase promoting complex subunit 1; anaphase-promoting complex 1 (meiotic checkpoint regulator); Anaphase-promoting complex subunit 1; Apc 1; APC1; APC1_HUMAN; Cyclosome subunit 1; MCPR; Meiotic checkpoint regulator; Mitotic checkpoint regulator; Protein Tsg 24; Protein Tsg24; Testis-specific gene 24 protein; TSG 24; TSG24. |
DATASHEET
Host:Rabbit
Target Protein:phospho-APC1 (Ser355)
IR:Immunogen Range:AH(p-S)PA
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:64682
Swiss Prot:Q9H1A4
Source:KLH conjugated Synthesised phosphopeptide derived from human ANAPC1 around the phosphorylation site of Ser355:AH(p-S)PA
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:This gene encodes a subunit of the anaphase-promoting complex. This complex is an E3 ubiquitin ligase that regulates progression through the metaphase to anaphase portion of the cell cycle by ubiquitinating proteins which targets them for degradation. [provided by RefSeq, Dec 2011].
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Rabbit
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Ovary (Mouse) Lysate at 40 ug
Primary: Anti- phospho-APC1 (Ser355) (bs-5173R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 212 kD
Observed band size: 212 kD
Paraformaldehyde-fixed, paraffin embedded (rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-APC1 (Ser355)) Polyclonal Antibody, Unconjugated (bs-5173R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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