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Anti-phospho-IL-1R1 (Tyr496) antibody (bs-5394R)

Anti-phospho-IL-1R1 (Tyr496) antibody (bs-5394R)

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Anti-phospho-IL-1R1 (Tyr496) antibody

英文名称phospho-IL-1R1 (Tyr496)
中文名称磷酸化白介素1受体1抗体
别    名IL1 Receptor I (phospho Tyr496); Antigen CD121a; CD121 antigen-like family member A; CD121A; CD121a antigen; D2S1473; IL 1; IL 1R 1; IL 1R alpha; IL 1RT1; IL-1R-1; IL-1R-alpha; IL-1RT-1; IL-1RT1; IL1 inhibitor; IL1R; IL1R1; IL1R1_HUMAN; IL1RA; IL1RN; IL1RT1; Interleukin 1 receptor alpha type 1; Interleukin 1 receptor antagonist protein (IRAP); Interleukin 1 receptor type I; Interleukin receptor 1; Interleukin-1 receptor alpha; Interleukin-1 receptor type 1; Interleukin-1 receptor type I; P80; IL1 Receptor I; IL-1 Receptor I; Interleukin-1 receptor type 1, soluble form; IL-1 sRI.  

DATASHEET

Host:Rabbit

Target Protein:phospho-IL-1R1 (Tyr496)

IR:Immunogen Range:QD(p-Y)EK

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:3554

Swiss Prot:P14778

Source:KLH conjugated Synthesised phosphopeptide derived from human IL-1R1 around the phosphorylation site of Tyr496:QD(p-Y)EK 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein is a receptor for interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA). It is an important mediator involved in many cytokine induced immune and inflammatory responses. This gene along with interleukin 1 receptor, type II(IL1R2), interleukin 1 receptor-like 2 (IL1RL2), and interleukin 1 receptor-like 1 (IL1RL1) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(1μg/Test)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Horse
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample: Intestine (Mouse) Lysate at 40 ug
Primary: Anti-phospho-IL-1R1(Tyr496) (bs-5394R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 63 kD
Observed band size: 63 kD

Blank control (blue line): MCF7 (fixed with 70% methanol overnight at 4℃).
Primary Antibody (green line): Rabbit Anti-phospho-IL-1R1(Tyr496)antibody (bs-5394R),Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.

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