Anti-phospho-HDAC1 (Ser423+Ser421) antibody
| 英文名称 | phospho-HDAC1 (Ser423+Ser421) |
| 中文名称 | 磷酸化组蛋白去乙酰化酶1抗体 |
| 别 名 | HDAC1 (phospho S421 + S423); p-HDAC1 (phospho S421 + S423); dHDAC1 (phospho S421 + S423); p-dHDAC1 (phospho S421 + S423); DKFZp686H12203; GON 10; HD 1; HD1; HDAC 1; hdac1: histone deacetylase 1; Histone deacetylase 1 (HD1); Histone deacetylase 1; RPD3 (reduced potassium dependency yeast homolog) like 1; RPD3 (reduced potassium dependency); RPD3; RPD3L1; HDAC2_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:phospho-HDAC1 (Ser423+Ser421)
IR:Immunogen Range:EF(p-S)D(p-S)E
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:3065
Swiss Prot:Q13547
Source:KLH conjugated Synthesised phosphopeptide derived from human HDAC1 around the pho sphorylation site of Ser423+Ser421:EF(p-S)D(p-S)E
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq, Jul 2008]
Size:100ul
Concentration:1mg/ml
Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Bone (Mouse) Lysate at 40 ug
Primary: Anti-phospho-HDAC1 (Ser423+Ser421) (bs-5388R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-HDAC1 (Ser423+Ser421)) Polyclonal Antibody, Unconjugated (bs-5388R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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