Anti-phospho-MEK5 (Ser137) antibody
| 英文名称 | phospho-MEK5 (Ser137) |
| 中文名称 | 磷酸化丝裂原活化蛋白激酶激酶5抗体 |
| 别 名 | MAP2K5(phospho Ser137); MAP2K5(phospho S137); Dual specificity mitogen activated protein kinase kinase 5; Dual specificity mitogen-activated protein kinase kinase 5; EC 2.7.12.2; HsT17454; MAP kinase kinase 5; MAP kinase kinase MEK5b; MAP2K5; MAPK/ERK kinase 5; MAPKK 5; MAPKK5; MEK 5; mitogen-activated protein kinase kinase 5; MKK5; MP2K5_HUMAN; PRKMK5; Protein kinase, mitogen-activated, kinase 5; SAPKK5; SKK5. |
DATASHEET
Host:Rabbit
Target Protein:phospho-MEK5 (Ser137)
IR:Immunogen Range:AV(p-S)DS
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:5607
Swiss Prot:Q13163
Source:KLH conjugated Synthesised phosphopeptide derived from human MEK5 around the phosphorylation site of Ser137:AV(p-S)DS
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:MEK5 is a dual specificity protein kinase belonging to thr Ser/Thr protein kinase family, (MAP kinase kinase family). It is activated by phosphorylation on Ser/Thr by MAP kinase kinases and interacts specifically with ERK5, and not with another MAP kinase like P38. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). It is not phosphorylated by RAFA, RAFB or RAFC and it may interact with GTPases such as CDC42. The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. MEK5 is expressed in many adult tissues and is most abundant in heart and skeletal muscle.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Cow
Horse
Rabbit
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample: MDA-MB-231 Cell (Human) Lysate at 40 ug
Primary: Anti-phospho-MEK5(Ser137) (bs-5432R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 50 kD
Sample: Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti- phospho-MEK5(Ser137) (bs-5432R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 50 kD
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