Anti-phospho-MEF2C (Thr20) antibody
| 英文名称 | phospho-MEF2C (Thr20) |
| 中文名称 | 磷酸化肌细胞增强因子2C抗体 |
| 别 名 | MEF2C(phospho Thr20); MEF2C(phospho T20); MADS box transcription enhancer factor 2 polypeptide C (myocyte enhancer factor 2C); Myocyte enhancer factor 2C; Myocyte specific enhancer factor 2C; Similar to MADS box transcription enhancer factor 2 polypeptide C; MEF2C_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:phospho-MEF2C (Thr20)
IR:Immunogen Range:QV(p-T)FT
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:4208
Swiss Prot:Q06413
Source:KLH conjugated Synthesised phosphopeptide derived from human MEF2C around the phosphorylation site of Thr20:QV(p-T)FT
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:MEF2C is a transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. This protein controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. It may also be involved in neurogenesis and in the development of cortical architecture.
Size:100ul
Concentration:1mg/ml
Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Rabbit
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-MEF2C(Thr20)) Polyclonal Antibody, Unconjugated (bs-5482R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-MEF2C(Thr20) Polyclonal Antibody, Unconjugated(bs-5482R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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